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Chip Technology by Springer-Verlag, Jorg Hoheisel

By Springer-Verlag, Jorg Hoheisel

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Embedded Systems Firmware Demystified

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- like any unmarried CMP (fomerly R&D) publication, this one is very poorly written and activities a large number of ridiculous typos that
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Reprinted with permission from Warrington JA et al (2000) In: Microarray biochip technology. H. C. Christians phisms. Because SNPs are the most common source of variation between individuals, they serve not only as landmarks to create dense genome maps, but also as markers for linkage and loss of heterozygosity studies. Large numbers of publicly available SNPs – over one million to date – have been found using dideoxy sequencing as well as mutation detection arrays [41–43]. In addition to mutation detection arrays, at least two other types of oligonucleotide arrays can be used for SNP analysis.

Milner et al. [8] used β-globin mRNA as target and showed that duplex yield on a scanning array of anti-β-globin antisense oligonucleotides correlated with their inhibitory potential in an in vitro translation assay. We have also tested the antisense activities of over 30 oligonucleotides targeted to mRNAs of cyclins B1, B4 and B5 of Xenopus laevis in cell-free egg extracts and oocytes [17]. Scanning arrays corresponding to the first 120 nucleotides of each of the three B-type cyclins were hybridised to their corresponding transcript.

9). This process has provided stepwise synthetic yields >98%, photolysis speeds at least an order of magnitude faster than that achievable with photo-removable protecting groups, and photolithographic resolution capability well below 10 microns. This technology will enable the production of arrays with much higher information content than is currently attainable. High-Density GeneChip Oligonucleotide Probe Arrays 31 Flexible Custom Array Fabrication. Another recent development has been the application of programmable digital micromirror devices, or “digital light processors” (DLPs), for photolithographic imaging, which offers a flexible approach to custom photolithographic array fabrication [21].

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